Invitrogen™

DES™-Inducible Kit with pCoBlast

The DES™-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector자세히 알아보기

The DES™-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT/V5-His vector offers the following features:

• Small size (3.5 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogen`s Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

사양

프로모터MT

제품 유형Inducible Kit

단백질 태그 위치(유전자에 대해)C-terminal

단백질 태그His Tag (6x), V5 Epitope Tag

클로닝 방법Gateway

수량1 kit

벡터pMT

제품라인DES™

Unit SizeEach